Medical Biotechnology

Equipment

Department Cryo & Stem Cell Technology

Pluripotency & Regeneration, Automation Processes and Cryobiotechnology

  • Cell biology laboratory, equipped with:
    - flow and laminar hoods
    - CO2 incubators
    - 10 BioLevitators (semi-automated suspension cell culture bioreactor)
    - bioreactor systems for the expansion of cells (spinner flasks, hexabatch)
    - 10 BioStations IM and 1 Biostation CT for low-content time-lapse imaging
    - FACSAria II flow cytometer and cell sorter
    - separate quarantine cell culture laboratory for primary cells
  • Molecular biology laboratory, equipped with:
    - QuantStudio 7 qPCR instrument for quantitative gene expression studies
    - Thermalcycler (AB 2720)
    - Bioanalyzer and Nanodrop for precise measurement of nucleic acids and protein concentration
    - electrophoresis systems for agarose gels and SDS-PAGE
    - equipment for recombinant DNA techniques
  • Biochemistry laboratory, equipped with:
    - hybridization oven for in situ detection of RNA
    - BioRad Chemidoc for quantification of nucleic acid/protein expression
    - Thermalcycler (AB SimpliAmp)
    - spectrophotometer
    - microcapsule/microcarrier producing system (co-axial air flow and Crystal Gun principle)
    - contact printer (GeSiM GmbH) a moulding system for the production of thin, biocompatible alginate films or for printing of protein patterns
    - 3D scaffolder (GeSiM GmbH) for the production of three dimensional scaffolds for tissue engineering
    - freeze dryer
  • Microscopy room, equipped with:
    - combined reflection/scanning force microscope for measurement of biological objects in aqueous environments
    - confocal inverted microscope
    - fluorescence microscopes, CLSM, LSM, incubation microscopes
  • Automation laboratory, equipped with:
    - cell culture robots for the automated cultivation of cells:
        - Nanoplotter 2.1 (GeSiM GmbH)
        - TECAN Freedom 200 (TECAN)
        - TAP arm (TAP BIOSYSTEMS)
    - micropipette system / automation platform
  • Cryopreservation laboratory, equipped with:
    • cryogenic storage systems (down to -196 °C) with liquid nitrogen storage tank
    • automated cryobanking system, currently with the capacity of 60,000 samples (hermetic storage, ASKION)
    • Cryo-Workbench (ASKION) for the handling of frozen samples below -100°C
    • vitrification facility with novel substrates for sterile vitrification processes
    • computer-controlled freezers (Sylab, Asymptote)
    • cryomicroscope including high-speed camera
    • "Freezing Spin Coater" for the freezing of ultrathin layers (own development)
    • modified programmable automatic freezer for applications in biology, materials science and electronics
    • high-speed camera system for analysis of fast biological and biophysical processes (e.g. microdrop-based freezing)
    • thermography system (temperature measurement range –20 °C to +250 °C)
    • thawing device for plasma bags
    • cryogenic osmometer (Gonotec)


Biomedical Optics

  • ultra-short pulsed Ti:sapphire laser, various additional pulsed and cw laser sources
  • multiphoton laser scanning microscope with spectral imaging module (Zeiss LSM510-Meta-NLO)
  • epifluorescence microscope with CCD unit
  • versatile platform for development and evaluation of laser scanning imaging technology (established as standard: confocal fluorescence and Raman microscopy at 375, 532 and 785 nm excitation, multiphoton microscopy at 710-990 nm excitation, detection by 1024-channel spectrograph, option for subsequent correlation of measured values)
  • ultra-short pulse technology: pulse picker, frequency doubler, beam analysis systems
  • low-temperature microscopy equipment
  • equipment for time-correlated single photon counting (TCSPC) for fluorescence lifetime imaging (FLIM) and spectral FLIM
  • measurement electronics (pulse generators, oscilloscope, lock-in amplifier, etc.)
  • fluorescence spectrometer (200-900 nm), UV/Vis/NIR absorption spectrometer (200-3300 nm)
  • Peltier-cooled CCD spectrometer (Andor Idus)
  • hardware correlator ALV-5000
  • piezotables for various control ranges
  • spin coater
  • differential scanning calorimeter Perkin Elmer DSC 8500 (-180 °C to +750 °C)
  • Lab View development environment
  • Zemax: optical design software
  • clean room for the extraction of high-purity alginates
  • measurement station for the deformation curves of highly viscous alginate

 

Department Bioprocessing & Bioanalytics

Laboratories with safety level S-2 and S-3 with double-door system for microbiological, molecular biological and cell biological research as well as radionuclide laboratory of safety level S2 for use with open radioactive substances

  • aerosol exposition system VITROCELL® Cloud (air-liquid-interface model, e. g. lung barrier
  • TER impedance measuring system (cellZscope®, Ussing chamber®) for transport studies and barrier function analysis of liquid-liquid-interfaces
  • Franz cell system® (air-liquid-interface model, e. g. skin barrier)
  • flow cytometer including sorting unit (FACS)
  • spectral photometer for absorption, fluorescence and luminescence measurements of microtitre plates
  • transmitted light microscopes and reflected light microscopes with phase and differential interference contrast, fluorescence unit, manipulation unit and incubation hood, sample stage variable in the z-axes for the 3D display of biological samples
  • Leica-TCS-SP8-X confocal microscope equipped with white-light laser and continuous wave laser with a wavelength of 405 nm (white-light-CLSM)
  • field-flow-fractionation system (AF2000 MultiFlow FFF) with UV and MALS detector
  • high-performance liquids chromatograph (HPLC)
  • Zetasizer Nano
  • NanoSight – Nanoparticle Tracking Analysis
  • gel electrophoresis units for DNA, RNA and proteins (with documentation units)
  • Western Blot unit
  • real-time PCR cycler
  • cryo microtome
  • micro-, cooling and ultracentrifuges
  • fluid scintillation counter model 2919 TR

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