Cryo & Stem Cell Technology

Offers

Pluripotency & Regeneration

  • research and development of new cell-based screening methods with the focus on miniaturization, parallelization and automation
  • development of innovative concepts for the automation of cell culture processes in the field of stem cell research
  • research on the control of pluripotency in an adult body using the unique features of the free-living flatworm S. mediterranea
  • investigation of high-density expansion of hiPSCs cultured in suspension on alginate microcarriers
  • integration of complete cell culture processes in automation technologies using robotics and microfluidics:
    - automated reprogramming
    - automated hiPSC differentiation
  • development of standardized QC for the rapid and consistent evaluation of the pluripotential of hiPSCs
  • development of innovative analytical methods for the investigation of three-dimensional cell constructs  
  • cell and tissue engineering of medically relevant systems using biocompatible hydrogels
  • immunoisolation of medically relevant cell systems using biocompatible hydrogels
  • development of an innovative bag-based culture system for the differentiation and cryopreservation of pluripotent stem cells


Biomedical Optics

  • confocal and non-linear laser scanning microscopy for tasks in biomedical and materials science (fluorescence and Raman, multiphoton excitation, second harmonic imaging)
  • optical spectroscopy (UV/Vis/Nir absorption, fluorescence, Raman)
  • laser scanning cryomicroscopy and cryogenic calorimetry
  • laser-based 3D micro and nanostructuring of polymers, metal films, silicon and biological material
  • fluorescence lifetime imaging (FLIM), spectrally resolved fluorescence lifetime imaging (S-FLIM)
  • concepts for functional optical sensors and imaging 
  • design of miniaturized scanners and optics 
  • conception and execution of optical-microscopic studies on cells, cell assemblies, tissues and non-biological samples (confocal, nonlinear, transmission, fluorescence) for biology, pharmacy and materials science
  • conception and execution of optical-spectroscopic studies (UV/Vis/NIR)
  • application and evaluation of molecular probes for imaging of physical, chemical and biological environmental parameters in biomedicine and non-biological application fields
  • application and evaluation of optical biomarkers (contrast agents, molecular imaging) for diagnostics, monitoring and research
  • development and adaptation of optical sensor concepts and architectures
  • development and adaptation of imaging optical contrast processes for biomedicine and materials science
  • 3D spatially resolved photochemistry: photopolymerization, uncaging etc.
  • ablative laser micro processing
  • fluorescence-spectroscopic measurements (200-900 nm)
  • absorption-spectroscopic measurements (200-3300 nm)
  • laser scanning microscopy: confocal reflexion and fluorescence, multiphoton microscopy
  • SHG microscopy for specific and marker-free representation of collagen, starch, myosine, etc.
  • wide field microscopy
  • development of imaging methods for long-term time-lapse monitoring of biological systems using automated image analysis
  • characterization of biopolymers (e. g. alginate) for cell culture of therapeutically relevant cell systems
  • development of stimuli-responsive surfaces for cell culture of therapeutically relevant cell systems


Automation Processes

  • automated biobanking of biological material, micro-organisms and clinical samples under BSL 2 according to the genetic engineering act, infection protection act, biological agents regulations (DIN EN ISO9001:2015)
  • optimization and validation of automated cell culture processes
    • cultivation of cell lines and induced pluripotent stem cells
    • production von HIV-1 pseudo-type viruses (“Good Clinical Laboratory Practice” (GCLP) - conform)
    • reprogramming und differentiation of induced pluripotent stem cells
  • automation of cell-based assays and processes
    • cytotoxicity assays, neutralization assays, ELISpot, embryonic stem cell assay, aliquoting, cryopreservation
    • optimization and validation up to certification under a quality management system


Cryobiotechnology

  • Research and development of novel cell culture and screening systems with focus on miniaturization, parallelization and automation
  • development of new concepts for automation of cell culture procedures in the area of stem cell research
  • integration of complete cell culture processes of automation technologies using robotics and microfluidics
    • automated toxicity testing
    • automated hiPSC differentiation
  • development of novel analysis methods for the investigation of three-dimensional cell constructs under miniaturized conditions
  • research and development in the area of cryobiology and biotechnology
    • development of novel sterile vitrification protocols
    • development of new vitrification substrates
    • development of chemical defined high performance cryo media
  • cell and tissue-specific optimization of cryopreservation processes
    • vitrification of hiPSCs on alginate microcarriers
    • cryopreservation of multicellular constructs
    • cryopreservation of adherent cell systems
  • development of optimized disposables for cultivation, manipulation and live storage of viable cells and tissue at cryogenic temperatures

 

Equipment of the Department of Cryo & Stem Cell Technology

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