Cryo & Stem Cell Technology

Equipment

Pluripotency & Renereration, Automation Processes & Cryobiotechnology

Cell biology laboratory, equipped with:

  • flow and laminar hoods
  • CO2 incubators
  • 10 BioLevitators (semi-automated suspension cell culture bioreactor)
  • bioreactor systems for the expansion of cells (Spinner Flasks, Hexabatch)
  • 10 BioStations IM and 1 Biostation CT for low-content time-lapse imaging
  • FACSAria II flow cytometer and cell sorter
  • separate quarantine cell culture laboratory for primary cells

Molecular biology laboratory, equipped with:

  • QuantStudio 7 qPCR instrument for quantitative gene expression studies
  • Thermalcycler (AB 2720)
  • Bioanalyzer and Nanodrop for precise measurement of nucleic acids and protein concentration
  • electrophoresis systems for agarose gels and SDS-PAGE
  • equipment for recombinant DNA techniques

Biochemistry laboratory, equipped with:

  • hybridization oven for in situ detection of RNA
  • BioRad Chemidoc for quantification of nucleic acid/protein expression
  • Thermalcycler (AB SimpliAmp)
  • spectrophotometer
  • microcapsule/microcarrier producing system (co-axial air flow and Crystal Gun principle)
  • contact printer (GeSiM GmbH) a moulding system for the production of thin, biocompatible alginate films or for printing of protein patterns
  • 3D scaffolder (GeSiM GmbH) for the production of three dimensional scaffolds for tissue engineering
  • freeze dryer

Microscopy room, equipped with:

  • inverted cryomicroscope (own development, Peltier-based)
  • combined reflection/scanning force microscope for measurement of biological objects in aqueous environments
  • confocal inverted microscope
  • fluorescence microscopes, CLSM, LSM, incubation microscopes
  • high-speed camera for the visualization of ultra-fast processes like crystallization during cryopreservation or the validation of cross-linking processes

Automation laboratory, equipped with:

  • cell culture robots for the automated cultivation of cells:
    - Nanoplotter 2.1 (GeSiM GmbH)
    - TECAN Freedom 200 (TECAN)
    - TAP arm (TAP BIOSYSTEMS)
  • micropipette system / automation platform

Cryopreservation laboratory, equipped with:

  • cryogenic storage systems (down to –196 °C) with liquid nitrogen storage tank
  • automated cryobanking system, currently with the capacity of 60,000 samples (hermetic storage, ASKION)
  • Cryo-Workbench (ASKION) for the handling of frozen samples below -100°C
  • vitrification facility with novel substrates for sterile vitrification processes
  • computer-controlled freezers (Sylab, Asymptote)
  • cryomicroscope including high-speed camera
  • "Freezing Spin Coater" for the freezing of ultrathin layers (own development)
  • modified programmable automatic freezer for applications in biology, materials science and electronics
  • high-speed camera system for analysis of fast biological and biophysical processes (e.g. microdrop-based freezing)
  • thermography system (temperature measurement range –20 °C to +250 °C)
  • thawing device for plasma bags
  • cryogentic osmometer (Gonotec)


Biomedical Optics

  • ultra-short pulsed Ti:sapphire laser, various additional pulsed and cw laser sources
  • multiphoton laser scanning microscope with spectral imaging module (Zeiss LSM510-Meta-NLO)
  • epifluorescence microscope with CCD unit
  • versatile platform for development and evaluation of laser scanning imaging technology (established as standard: confocal fluorescence and Raman microscopy at 375, 532 and 785 nm excitation, multiphoton microscopy at 710-990 nm excitation, detection by 1024-channel spectrograph, option for subsequent correlation of measured values)
  • ultra-short pulse technology: pulse picker, frequency doubler, beam analysis systems
  • low-temperature microscopy equipment
  • equipment for time-correlated single photon counting (TCSPC) for fluorescence lifetime imaging (FLIM) and spectral FLIM
  • measurement electronics (pulse generators, oscilloscope, lock-in amplifier, etc.)
  • fluorescence spectrometer (200-900 nm), UV/Vis/NIR absorption spectrometer (200-3300 nm)
  • Peltier-cooled CCD spectrometer (Andor Idus)
  • hardware correlator ALV-5000
  • piezotables for various control ranges
  • spin coater
  • differential scanning calorimeter Perkin Elmer DSC 8500 (-180 °C to +750 °C)
  • Lab View development environment
  • Zemax: optical design software
  • clean room for the extraction of high-purity alginates
  • measurement station for the deformation curves of highly viscous alginate

 

Offers of the Department of Cryo & Stem Cell Technology

Brochure of Main Department of Medical Biotechnology

Product Information Sheets of the Main Department of Medical Biotechnology

Projects of the Main Department of Medical Biotechnology

All Publications of the Main Department of Medical Bioechnology

Press Releases of the Main Department of Medical Biotechnology

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